The SEAP (Secreted Embryonic Alkaline Phosphatase) is commonly used as a powerful reporter gene whose signal can be detected via chemiluminescent reporter assay based on dioxetane CSPD (chloro-5-substituted adamantyl-1,2-dioxetane phosphate), and provides a convenient and highly sensitive method for the quantitation of transcriptional activity. SEAP secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters, like luciferase. It allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies.
This lentivirus express SEAP reporter under a minimal CMV promoter that inserted with 8 tandem repeats of CRE_TRE (cAMP Response Element_Transcription Response Element), for detecting or monitoring cAMP-PKA signaling pathway activity.
This lentivirus also contain the Red Fluorescent protein (RFP) marker under the constitutive RSV promoter for antibiotic selection. See Product Manualfor details (.pdf).
If desired, you can also use a pathway “Control Lentivirus” to establish the No-response control profile to your pathway stimulus. The corresponding pathway control lentivirus is: CAT#: Path-Ctr22
Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium containing 10x polybrene.