Three shRNA lentiviectors (5.0 ug/each) and three pre-made shRNA expression lentivirus (0.5 ml/each). One negative control shRNA vector (5.0ug) and virus (0.5ml).
The shRNA was expressed under an optional Tetracycline inducible human H1 promoter. It can be used as constitutive expression of shRNA without any induction, or used as tetracycline inducible expression when the Tet-repressor protein is present in advance. The shRNA lentivector aslo contains a RFP-Puromycin fusion dual marker under Rsv promoter, which allows to select the shRNA positive cell by Puromycin antibiotic selection or by FACS cell sorting via RFP signal. See shRNA cloning kit manual for more details about the shRNA lentivector.
Order will be delivered within 2 weeks.
Amounts: (1) Plasmid DNAs: 5 ug/each x 4 tubes; (2): pre-made shRNA virus: 0.5ml/each x 4 tubes. (vectors and viruses: three target specific shRNA and one negative control shRNA).
Note: please provide the target accession number during checkout in the “Comments” field.