Description
In mammals, DNMT1, DNMT3a and DNMT3b, are the generally recognized three types of DNA methyltransferases (DNMTs). They add the methyl group to DNA (DNA methylation process) and silence gene expression. The dCas9-DNMTs system offers unrivaled applications in alter the methylation of specific genes [Reference], and became a powerful epigenetic editing tool for gene repression.
This premade lentivirus express the dCa9-DNMT3a fusion under the enhanced CMV promoter (SuCMV), carrying Blasticidin selection. It can be used to silence the desired genes (for example, methylation of desired promoter), or generate a genome-wide repression-ready cell line, which can then be used for the targeted silencing upon gRNA addition.
If desired, the negative control lentivirus, CAT#: LVP1595, which has the dCas9 fusioned with a Null sequence, can be used as the non-specific, background control.
To repress your desired gene, simply apply this lentivirus to your cells and then add the targeting gRNA lentivirus (which need to be designed and made separately according to your target locus.), or apply this lentivirus together with your desired gRNA lentivirus. (Note: Contact us if you need to make your desired gRNA lentivirus via a customized order).
see details in Product Manual.
Amount: 200 ul/vial, at titer of 1×108 IFU/ml, provided in PBS solution (premixed with 10x polybrene, 60 ug/ml).
CAT#: LVP1551
