The human heat shock factor 1 (HSF1) is a transcription factor. The “dCas9-HSF1” fusion combines the DNA-targeting capabilities of dCas9 with the transcriptional activation function of HSF1. It can binds to the heat shock response elements (HSEs) in the promoter regions of genes, resulting in the transcriptional upregulation of the targeted genes [Reference]. The activity of HSF1 can be regulated by external stimuli such as heat shock or chemical inducers [ Reference ]. By using the dCas9-HSF1 fusion, researchers can achieve inducible and reversible control of gene expression.
This premade lentivirus express the dCa9-P65 fusion under the enhanced CMV promoter (SuCMV), carrying Puromycin selection. It can be used to activate the desired genes, or generate a genome-wide activation-ready cell line, which can then be used for the targeted gene-expression activation upon gRNA addition for screening and profiling assay.
If desired, the negative control lentivirus, CAT#: LVP1594, which has the dCas9 fusioned with a Null sequence, can be used as the non-specific, background control.
To up-regulate your desired gene, simply apply this lentivirus to your cells and then add the targeting gRNA lentivirus (which need to be designed and made separately according to your target locus.), or apply this lentivirus together with your desired gRNA lentivirus. (Note: Contact us if you need to make your desired gRNA lentivirus via a customized order).
see details in Product Manual.
Amount: 200 ul/vial, at titer of 1×108 IFU/ml, provided in PBS solution (premixed with 10x polybrene, 60 ug/ml).