KRAB ((Krüppel-associated box) is protein domain is known for its ability to repress the activity of nearby genes by recruiting chromatin-modifying enzymes to the DNA[Ref.], acting as a transcriptional repressor. The dCas9-KRAB system is a powerful tool for transcriptional repression of genes within their endogenous genomic loci.
This premade lentivirus express the dCa9-KRAB fusion under the enhanced CMV promoter (SuCMV). It does not contain any antibiotic selection. It can be used to silence the desired genes, targeted upon gRNA addition.
If desired, the negative control lentivirus, CAT#: LVP1597, which has the dCas9 fusioned with a Null sequence, can be used as the non-specific, background control.
To repress your desired gene, simply apply this lentivirus to your cells and then add the targeting gRNA lentivirus (which need to be designed and made separately according to your target locus.), or apply this lentivirus together with your desired gRNA lentivirus. (Note: Contact us if you need to make your desired gRNA lentivirus via a customized order).
see details in Product Manual.
Amount: 200 ul/vial, at titer of 1×108 IFU/ml, provided in PBS solution (premixed with 10x polybrene, 60 ug/ml).