Pre-made Lentivirus expressing Epstein Barr Virus (EBV) nuclear antigen 1 gene, EBNA1 (MG182339.1), under enhanced EF1a promoter. A GFP–Puromycin Fusion dual marker was expressed under RSV promoter, which allows to select the transduced cell via Puromycin antibiotic or via cell sorting by GFP fluorescent signal.
It is used for cell immortalization. See Product Manual (.pdf). Note: The EBNA1 gene is also enable high-copy episomal replication of any oriP embedded plasmids for high level gene expression.
Amount: This is the concentrated lentivirus, provided in PBS solution as 200ul/per vial at 1 x 108 IFU/ml, best used for serum free cell culture, hard-to-infected cell types, or In Vivo application.