Cas9 (EF1a, GFP-Puro) lentiviral particles

$295.00

SKU: LVP708 Categories: ,

Description

Lentivirus express human codon-optimized Cas9 endonuclease (Streptococcus pyogenes )  under the enhanced EF1a promoter, with a proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains a GFP-Puromycin fusion  Fluorescent-antibiotic dual marker for the selection of the transduced cells. (see also the Non-Integration Lentivirus of this product, CAT#: IDLV007B.)

See Product Manual here (.pdf).

Application: used for CRISPR gene editing ( Note: used with a separate “gene-specific gRNA lentivirus” or ” gene-specific gRNA vector” for CRISPR editing )

Amount:  5 x 106 IFU/ml x 200ul in DMEM medium with 10% FBS, 10x of polybrene. 

Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.

Cat#: LVP708