Lentivirus express human codon-optimized Cas9 endonuclease (Streptococcus pyogenes ) under the enhanced EF1a promoter, with a proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains a Puromycin antibiotic marker for the selection of the transduced cells. (see also the Non-Integration Lentivirus of this product, CAT#: IDLV004B.)
This is the concentrated lentivirus with higher titer. See Product Manual here (.pdf).
Application: used for CRISPR gene editing ( Note: used with a separate “gene-specific gRNA lentivirus” or ” gene-specific gRNA vector” for CRISPR editing )
Amount: 5 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 10x of polybrene.
Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.