EF1a-h Cas9 (Puro) lentiviral particles


SKU: LVP684 Categories: ,


Pre-made lentivial particles express human codon optimized wild-type Cas9 endonuclease  under an enhanced EF1a promoter with our proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains a Puromycin antibiotic marker for selection of the transduced cells. (see also the Non-Integration Lentivirus of this product, CAT#: IDLV004B.)

See Product Manual here (.pdf).

Application: used for CRISPR gene editing ( Note: used with a separate “gene specific gRNA lentivirus” or ” gene specific gRNA vector” for CRISPR editing )

Amount:  5 x 106 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene. 

Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.

Cat#: LVP684