Pre-made lentivial particles express human codon optimized wild-type Cas9 endonuclease under an enhanced EF1a promoter, with our proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains a RFP-Puromycin fusion dual marker (under RSV promoter) for selection of the transduced cells via RFP fluorescent signal or via Puromycin antibiotic. The fluorescent signal provides a real-time monitoring of the lentivirus’ transduction efficiency in your cell types.
See Product Manual here (.pdf).
Application: used for CRISPR gene editing ( Note: used with a separate “gene specific gRNA lentivirus” or ” gene specific gRNA vector” for CRISPR editing )
Amount: 5 x 106 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.
Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.