Pre-made firefly Luciferase (luc) Lentiviral particles expressing luciferase II gene under re-engineered EF1a promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically expressed as individual protein mediated by a 2A element. A Blasticidin antibiotic marker was expressed under RSV promoter, allows to sort or select transduced cells for long term expression via GFP signal or via blasticidin antibiotic (Dual selection). GFP signal provides a convenient, realtime monitoring the particles performance. See Product Manual for details (.pdf).
Amount: >200ul/per vial, at 1 x 107 IFU/ml in DMEM medium containing 10x polybrene.