Pre-made Lentivirus expressing luciferase II gene under a re-engineered EF1a promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically expressed as individual protein mediated by a 2A element. A Neomycin antibiotic marker was expressed under RSV promoter, allows to sort or select transduced cells via GFP signal or via Neomycin antibiotic (Dual selection). GFP signal provides convenient, realtime monitoring the performance of the lentivirus.
Lentivurs is concentrated and buffer exchanged into PBS as in vivo ready status. See Product Manual for details (.pdf).
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.