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Home / ImmunoOncology Research / Assay Cell Lines

Assay Cell Lines

gentarget's assay cell linesThe validation assays for the engineered T cells, are the most important step to obtain the best CAR-T in preclinical researches and clinical trials. Those assays include verifying the CAR gene expression (by Western blot, ELISA, Flow cytometry, or IHC), verifying the CAR’s immune responses (by cytokines release test, cyto-toxicity test, cell proliferation test), and verifying the CAR’s efficacy (by cell bases assay, T cell-mediated target cell lysis assay, and more).

To evaluate the targeted killing efficacy of CAR-T, Gentarget generates many targeted cell lines from different tumor cell types that are labeled with a fluorescent (GFP or RFP), or a tumor target (for example, CD19, PDL1  cell surface marker). When the target cells are co-cultured with the CAR engineered T cells, the target cells are killed and lost the fluorescent signal as results of cell lysis. The live cells can be imaged and monitored at real-time to measure the tumor cell lysis in Flow cytometry. It provides a fast, easy validation approach for CAR-T products. Those cell lines provide convenient, real-time monitoring of CAR efficacy and other pathway-specific reactions, in dose and time course matter.

Please see each cell line by clicking on each product page below.

NameSKUPriceBuy
A549 / Luciferase-2A-GFP (Puromycin) stable cellsSC043-LG$1,500.00
CHO / CD19 & GFP Expression Stable Cell LineSC086$4,000.00
CHO-GFP stable cells (Puromycin)SC039-Puro$700.00
GFP & RFP HEK293 stable cellsSC009$1,400.00
HEK293 / h PDL1 Expression Stable CellsSC083$1,500.00
HEK293 / human CD19 Expression Stable Cell LineSC087$3,000.00
Hela-GFP stable cells (Bsd)SC034-Bsd$650.00
Hela-nucGFP stable cellsSC070-G$1,000.00
Human 786-O (Luciferase / GFP) Stable CellsSC069-LG$3,500.00
Human AsPC1 (Luciferase & GFP) Stable CellsSC062-LG$1,500.00
Human B lymphocyte / GFP Stable CellsSC060-G$2,000.00
Human ES2 (Luciferase & GFP) Stable CellsSC066-LG$1,500.00
Human HT-29 / GFP Stable CellsSC080-G$2,500.00
Human MCF10A / GFP (Puro) Stable CellsSC073-GP$2,500.00
Human PANC-1 / (Luciferase & GFP) Stable CellsSC068-LG$2,000.00
Human SH-SY5Y / GFP stable cell lineSC042$550.00
Human SW1990 / GFP (Puro) Stable CellsSC074-GP$2,000.00
Human SW403 / GFP Stable CellsSC067-G$1,000.00
Human T47D / GFP Stable CellsSC072-G$1,500.00
Human U2OS / GFP Stable CellsSC084-G$2,500.00
Jurkat / GFP stable Cell Line (EF1a Promoter)SC049-1$2,000.00
Luciferase & GFP / HEK293 stable cells (Puro)SC002-GP$600.00
MDA-MB-231 / GFP (Puro) stable cell lineSC040-Puro$800.00
MLLB2 / GFP (Neomycin) stable cell lineSC055-G$1,000.00
Mouse CT26 / GFP Stable CellsSC061-G$1,000.00
Mouse CT26 / PDL1 Stable CellsSC061-PDL1$1,500.00
Mouse MB49 (Luciferase & GFP) Stable CellsSC065-LG$2,000.00
Mouse MB49 / GFP Stable CellsSC065-G$1,500.00
Mouse Panc02 / GFP Stable Cell LineSC078-G$3,000.00
RKO / GFP stable cells (Blasticidin)SC047-GB$800.00

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  • Home
  • Products
    • Premade Lentivirus
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    • Stable cell lines
    • Cell-Specific Reporter
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    • Non-integrating LV
    • Adenovirus
    • shRNA lentivirus
    • microRNA lentivirus
    • anti miRNA lentivirus
    • Lentivectors
    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
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    • Customized Stable Cell Lines
    • Fusion In Vivo PCR Cloning
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