Assay Cell Lines
The validation assays for the engineered T cells, are the most important step to obtain the best CAR-T in preclinical researches and clinical trials. Those assays include verifying the CAR gene expression (by Western blot, ELISA, Flow cytometry, or IHC), verifying the CAR’s immune responses (by cytokines release test, cyto-toxicity test, cell proliferation test), and verifying the CAR’s efficacy (by cell bases assay, T cell-mediated target cell lysis assay, and more).
To evaluate the targeted killing efficacy of CAR-T, Gentarget generates many targeted cell lines from different tumor cell types that are labeled with a fluorescent (GFP or RFP), or a tumor target (for example, CD19, PDL1 cell surface marker). When the target cells are co-cultured with the CAR engineered T cells, the target cells are killed and lost the fluorescent signal as results of cell lysis. The live cells can be imaged and monitored at real-time to measure the tumor cell lysis in Flow cytometry. It provides a fast, easy validation approach for CAR-T products. Those cell lines provide convenient, real-time monitoring of CAR efficacy and other pathway-specific reactions, in dose and time course matter.
Please see each cell line by clicking on each product page below.