Pre-made firefly Luciferase (luc) Lentiviral particles expressing luciferase II gene under a re-engineered EF1a promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically expressed as individual protein mediated by a 2A element. A Puromycin antibiotic marker was expressed under RSV promoter, allows to sort or select transduced cells for long term expression via GFP signal or via Puromycin antibiotic (Dual selection). GFP signal provides a convenient, realtime monitoring the particles performance.
Particles was concentrated and buffer exchanged into PBS as in vivo ready status. See Product Manual for details (.pdf).
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.