Pre-made Lentivirus express firefily Luciferase gene under re-engineered EF1a promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically expressed as individual protein mediated by a 2A element. A Puromycin antibiotic marker was expressed under RSV promoter, which allows to sort or to select transduced cells for long term expression via GFP signal or via Puromycin antibiotic (Dual selection). GFP signal provides convenient, real-time monitoring the performance of the lentivirus. See Product Manual for details (.pdf).
Amount: >200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS.