Pre-made lentivirus expressing firefly luciferase 3 gene under an enhanced EF1a promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically expressed as individual protein mediated by a 2A element. Note: this lentivirus does not contain any antibiotic selection marker to satisfy the requirement for non-antibiotic marker environment application. GFP signal provides a convenient, realtime monitoring the particles performance. See Product Manual for details (.pdf).
Amount: >200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS.