Concentrated Lentivirus express luciferase gene under a re-engineered EF1a promoter. A RFP marker was co-expressed under the same promoter. Luciferase and RFP were bicistronically expressed as individual protein mediated by a 2A element. A Puromycin antibiotic marker was expressed under RSV promoter, allowing to sort or select transduced cells for long-term expression via RFP signal or via blasticidin antibiotic (Dual selection). RFP signal provides a convenient, real-time monitoring the particle’s performance.
Particles was concentrated and buffer exchanged into PBS as in vivo ready status. See Product Manual for details (.pdf).
Amount: 200ul/per vial, at 1 x 108 IFU/ml in PBS.