Pre-made firefly Luciferase (luc) Lentiviral particles expressing luciferase gene under a re-engineered EF1a promoter. A RFP marker was co-expressed under the same promoter. Luciferase and RFP were bicistronically expressed as individual protein mediated by a 2A element. A Puromycin antibiotic marker was expressed under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual selection). RFP signal provides a convenient, realtime monitoring the particles performance.
Particles was concentrated and buffer exchanged into PBS as in vivo ready status. See Product Manual for details (.pdf).
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.