Pre-made optional inducible lentiviral particles for expressing human target: ERBB2 (alternative name: NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_004448.3.
Full sequence verified target is natively expressed under an optional inducible suCMV promoter (Note: it can also used as regular constitutive high expression without any inducation required). A GFP-Puromycin fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via GFP signal or via Puromycin antibiotic (Dual markers). GFPsignal provides a convenient, realtime monitoring the particles performance. (please also see the RFP-Blasticidin version of this product, CAT#: LVP504)
Each particles was validated in lot by lot basis and target expression is guaranteed. Particles were packaged in DMEM medium contain 10% FBS and 10 x Polybrene. See Product manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium.