Flp recombinase (RFP-Bsd) lentiviral particles

$295.00

SKU: LVP014 Categories: ,

Description

Pre-made lentivirus express Flp recombinase under tetracycline inducible suCMV promoter.  A RFPBlasticidin fusion dual marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic.  RFP signal provides a convenient, realtime monitoring the particles performance. See Product Manual (.pdf).

Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism’s DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp).

The 34bp minimal FRT site sequence has the sequence

5’GAAGTTCCTATTCtctagaaaGtATAGGAACTTC3′

Amount:  200ul/per vial, at 1 x 107 IFU/ml in DMEM medium containing 10x polybrene

Cat#: LVP014