Pre-made lentivirus express Flp recombinase under tetracycline inducible suCMV promoter. A RFP–Blasticidin fusion dual marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic. RFP signal provides a convenient, realtime monitoring the particles performance. See Product Manual (.pdf).
Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism’s DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp).
The 34bp minimal FRT site sequence has the sequence
Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium containing 10x polybrene