Pre-made lentiviral particles expressing a fusion target of (GFP-human Actin ORF). The sequence verified codon sequence of human beta actin (NCBI accession ID: NM_001101) was fusioned at its N-terminal with GFP protein, expressed under a consitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter. It can be used to visualize sub-cellular structure where the actin was targeted.
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Please see product manual for details.
Amount: 200ul/vial (1×108 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).