Lentivirus express a fusion target of (GFP–β-Actin). The human β-actin (NCBI accession ID: NM_001101) was fusioned, at its N-terminal, with GFP (Green Fluorescent Protein) marker, expressed under the enhanced suCMV promoter, containing a Puromycin antibiotic marker under the RSV promoter. It can be used to visualize sub-cellular structure where the actin was targeted.
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta, and gamma, constituent of the contractile apparatus. The β-actin is the primary component of the cytoskeleton.
Fluorescent labelled Actin provides a convenient tools to invest the dynamic mechanisms of stress fiber assembly and adaptation to strain.
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