Pre-made GFP lentiviral particles expressing codon optimized Green Fluorescent Protein (GFP) under suCMV promoter, carrying a Blasticidin selection marker under Rsv promoter. The following scheme showed the lentivector’s core structure:
The particles was concentrated and buffer exchanged into PBS. (See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create GFP expression stable cell line (via Blasticidin selection);
Amount: 1 x 108 IFU/ml x 200ul in PBS.