Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under the CAG promoter. A Neomycin (Neo) / G418 selection marker was express under Rsv promoter, which allows to select transduced fluorescent positive cells. See Product Manual (.pdf).
The CAG promoter ( a hybrid of the CMV enhancer and chicken beta-actin promoter) has the highest expression level in embryonic stem (ES) cells.
Amount: 5 x 107 IFU/ml x 200ul in PBS. (This is the concentrated virus in PBS.)