Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under suCMV promoter. It does not contain any antibiotic selection. So if desired, you can only sort the transduced fluorescent positive cells. See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create GFP expression stable cell line (via fluorescent sorting);
Amount:1 x 107 IFU/ml x 200ul in DMEM medium with 60ug/ml polybrene (10x).