Pre-made Lentivirus expressing codon optimized Green Fluorescent Protein (GFP) marker under suCMV promoter. A Zeocin antibiotic selection under Rsv promoter allows to select transduced fluorescent positive cells. The following scheme showed the lentivector expression cassette:
See Product Manual (.pdf).
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.