Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under the enhanced EF1a promtoer. It does not contain any antibiotic selection. So if desired, you can only sort the transduced fluorescent positive cells.
This is the concentrated lentivirus with higher titer, resuspended in PBS solution. See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create GFP expression stable cell line (via fluorescent sorting);
Amount:1 x 108 IFU/ml x 200ul in PBS solution.