Pre-made GFP lentivial particles expressing codon optimized GFP under engineered EF1a promoter. It contains a Puromycin antibiotic selection marker under Rsv promoter. See Product Manual for details (.pdf).
The re-engineered EF1a promoter is strong constitutive promoter. It is non-tissue specific, highly expressed in all cell types, and less likely be silenced after long-term culture.
- monitor lentivirus transduction efficiency for your cells of interest;
- stable cell line generation;
Amount: 1 x 108 IFU/ml x 200ul in PBS.