GFP-His-RFP (Bsd) Lentiviral particles

$295.00

Description

This premade lentivirus bicistronically express GFP-6His and RFP  expressed under the same suCMV promoter mediated by  F2A self-cleavage site. A Blasticidin marker was expressed under RSV promoter, allowing the selection of transduced cells. GFP was expressed with C-term His tag (Note: RFP is not fusion with GFP).

The following scheme showed the lentivecotor’s core structure:

Application:

  1. Used as  control lentiviral particles to monitor the transduction ability for your cell of interest;
  2. Visualize expression efficiency by GFP signal and trasnduction efficiency via RFP signal;
  3. Used for making dual fluorescent GFP and RFP stable cell line via Blasticidin selection or flurescent cell sorting;
  4. Purify mammalian expressed His-tagged GFP protein; 
  5. Others;

See product manual (.pdf) for details.

Amount: 200ul/tube, at 1 x 107 IFU/ml in DMEM medium with 10% FBS, 60ug/ml polybrene. 

Cat#: LVP002