This premade lentivirus bicistronically express GFP-6His and RFP expressed under the same suCMV promoter mediated by F2A self-cleavage site. A Blasticidin marker was expressed under RSV promoter, allowing the selection of transduced cells. GFP was expressed with C-term His tag (Note: RFP is not fusion with GFP).
The following scheme showed the lentivecotor’s core structure:
- Used as control lentiviral particles to monitor the transduction ability for your cell of interest;
- Visualize expression efficiency by GFP signal and trasnduction efficiency via RFP signal;
- Used for making dual fluorescent GFP and RFP stable cell line via Blasticidin selection or flurescent cell sorting;
- Purify mammalian expressed His-tagged GFP protein;
See product manual (.pdf) for details.
Amount: 200ul/tube, at 1 x 107 IFU/ml in DMEM medium with 10% FBS, 60ug/ml polybrene.