Pre-made lentiviral particles expressing a fusion target of (GFP-human P53). The sequence verified human P53 gene codon sequence was fusioned at its N-terminal with GFP protein, expressed under a constitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter.
Human P53 (NCBI accession ID:NM_000546) involvs in many signal pathway. The fluorescent labeled P53 can be used to visualize sub-cellular pathway where the P53 bound or targeted at.
Please see product manual for details.
Amount: 200ul/vial (1×108 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).