Pre-made GFP_lacZ fusion Lentiviral particles expressing GFP and full-length Beta galactosidase (lacZ) fusion gene under suCMV promoter. Both GFP and lacZ are fully functional. GFP-LacZ fusion was expressed with C-term His-tag. A RFP was bibicistronically expressed under the same suCMV promoter for titer monitoring. So they are triple signal particles, with GFP, RFP signal visulized via microscope and lacZ signal via staining. A blasticidin marker under PGK promoter allows to select stable cells for long term expression, see lentivector core map scheme below:
Particle was concentrated into PBS solution via ultra-centrifugation as in vivo ready status. See product manual (.pdf).
Amount: 200ul/per vial at 5 x 107 IFU/ml in PBS solution.