Lentivirus express a fusion of “GFP-NEFL”. The codon sequence of human “Neurofilament light chain” / NEFL (gene_synonym: CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110; NCBI ID: NM_001163436 ), was fusioned in frame with Green Fluorescent Protein (GFP) at its N-terminal, expressed under a constitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter.
The protein encoded by the NEFL is a biomarker that can be measured with immunoassays in cerebrospinal fluid and plasma and reflects axonal damage in a wide variety of neurological disorders. It is a useful marker for disease monitoring in amyotrophic lateral sclerosis, multiple sclerosis, Alzheimer’s disease and more recently Huntington’s disease.
see Product Manual for all fusion products.
Amount: 200ul/vial (1×107 IFU/ml), provided in DMEM medium, containing 60 ug/ml of Polybrene (10x).