Pre-made GFP lentiviral particles containing codon optimized Green Fluorescent Protein (GFP) marker under suCMV promoter. A Neomycin (G418) antibiotic selection under Rsv promoter allows to select transduced fluorescent positive cells.
See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create GFP expression stable cell line (via Neomycin selection);
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.