Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under suCMV promoter. A Puromycin gene under Rsv promoter allows to select transduced fluorescent positive cells. See Product Manual (.pdf).
The particles was concentrated and buffer exchanged into PBS.
- monitor lentivirus transduction efficiency for your cells of interest;
- create GFP expression stable cell line (via Puromycin selection);
Amount: 1 x 108 IFU/ml x 200ul in PBS.