Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under suCMV promoter. A Puromycin gene under Rsv promoter allows to select transduced fluorescent positive cells. See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create GFP expression stable cell line (via Puromycin selection);
Amount:1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene (10x).