Pre-made lentiviral particles expressing a fusion target of (GFP-HIV-1 TAT). The sequence verified TAT protein codon sequence from HIV-1 virus was fusioned at its N-terminal with GFP protein, expressed under a consitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter.
Tat protein from HIV-1 virus contains a protein transduction domain and nuclear localisation signal. It is known as a cell penetrating protein. The fluorescent labeled TAT can be used to visualize sub-cellular pathway where the TAT targeted at. This particles showed the fluorescent signal located in nucleus area.
Please see product manual for details.
Amount: 200ul/vial (1×108 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).