This particles was produced in our optional inducible lentiviral system. It expresses codon optimized, Green Fluorescent Protein (GFP) under tetracycline inducible TetCMV promoter. A Puromycin selection under the constitutive RSV promoter, allows to select transduced cells via Puromycin antibiotic. The following scheme showed its expression cassette structure:
GFP is constitutively expressed in high level without any induction. But optionally, GFP can be used as Tetracycline inducible expression in the presence of TetR protein (repressor). Please see Product manual for details.
GFP signal can be visualized under fluorescent microscope. The Puromycin is constitutively expressed and not affected by Tetracycline induction.
Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS with 10x polyberne.