Lentivirus expresses codon optimized Green Fluorescent Protein (GFP) under Ubiquitin C (Ubc) promoter. A Puromycin gene under Rsv promoter allows to select transduced fluorescent positive cells. The following scheme represents its core expression cassette structure. See Product Manual (.pdf).
The promoter of the Ubc gene contains putative heat shock elements (HSEs), which mediates UBC induction upon stress. It can stably drive a gene expression in hESCs.
- measure the Ubc promoter strength in your desired cell types;
- monitor lentivirus transduction efficiency for your cells of interest;
- create GFP expression stable cell line (via Puromycin selection);
- others: promoter and pathway research;
Amount: 1 x 108 IFU/ml x 200ul in PBS. (This is the concentrated lentivirus in PBS).