Lentivirus express a fusion target of (GFP-Zyxin). The codon sequence of human Zyxin gene was fusioned, at its N-terminal, with GFP (Green Fluorescent Protein), under an enhanced suCMV promoter, containing a Puromycin antibiotic marker under the RSV promoter.
Human Zyxin gene (NCBI accession ID: NM_001010972.1) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. The fluorescent labeled Zyxin can be used to visualize sub-cellular signal transduction pathway where the adhesion-stimulated gene expression and the cytoskeletal organization of actin bundles happened.
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Amount: 200ul/vial (1x10e7 IFU/ml), provided in DMEM medium, containing 60ug/ml of Polybrene (10x).