Pre-made lentivirus for expressing human target: AXL (alternative name: JTK11, UFO), 100% matched CDS region in NCBI ID: NM_001699. It is used for constitutive high expression of AXL, or for tetracycline inducible expression when its repressor (TetR) is present which can be purchased separately.
The target is natively expressed under optional inducible CMV promoter. A blasticidin–RFP fusion dual marker under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides a convenient, realtime monitoring the particles performance. Each particles was validated in lot by lot basis and target expression is guaranteed. Particles were packaged in DMEM medium containing 10% serum. (Note: concentrated virus in PBS, at titer of 10×108 IFU/ml is available upon requested specifically, at price of $650 USD/vial, 200ul.)
See Product manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium.