The target is expressed under the enhanced EF1a promoter, containing Puromycin selection. see the expression cassette in the scheme below:
The engineer EF1a promoter is less cell-type-dependent and not be silenced In vitro and In vivo, also strong in many primary cells and T cells, B cells, and more. The Puromycin provides fast killing selection to generate a stable cell line. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 108 IFU/ml in PBS with 10x of polybrene (60 ug/ml).