Pre-made Lentivirus expresses human target, CD28 (human CD28 molecule) (alternative name: TP44). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID:NM_006139.3.
The target is expressed with the C-terminal 6His tag under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A Blasticidin–RFP fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of transduced cells. The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance. See Product Manual for details (.pdf).
This is the concentrated lentivirus, provided in PBS solution at a higher titer, best suited for hard-to-transduced cell types, or for any In Vivo application.
Amount: 200ul, at 1 x 108 IFU/ml in PBS.