Description
This Pre-made Lentivirus expresses human target, DNMT1 (DNA methyltransferase 1 ) (alternative name: ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID:NM_001379. DNMT1 add the methyl group to DNA (DNA methylation process) and silence gene expression.
The full sequence-verified target is expressed under the enhanced EF1a promoter (which is best suite for the hard-to-transduced cell types and suspension cells, like T Cells, B Cells). It also contains a GFP-Puromycin dual selection marker. The GFP signal provides convenient, real-time monitoring of the particles’ performance. See Product Manual for details.
When desired, use the cognate Null-control Lentivirus (CAT#: EF1a-Null-GP-PBS) as the negative control, for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but express the same GFP-Puromycin selection marker. See Also the dCas9-DNMT1 fusion lentivirus , CAT#: LVP1561
Amount: 200ul, at 1 x 108 IFU/ml (Premixed with 10x Polybrene / 60ug/ml)
Cat#: LVP1608
