Lentivirus expresses human target: HAVCR2 (CD366) gene (hepatitis A virus cellular receptor 2), (Gene_Synonym: CD366; HAVcr-2; KIM-3; SPTCL; Tim-3; TIM3; TIMD-3; TIMD3), with coding sequence 100% matching to CDS region in NCBI ID: NM_032782. It contains a RFP–Blasticidin dual selection marker.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details) with C-terminal 6His-tag. It constitutively expresses the target. Also optionally, It can be used for the inducible expression when used together with its repressor (TetR). An RFP-Blasticidin fusion dual selection marker under RSV promoter that allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides convenient, real-time monitoring of the performance of the particles.
When desired, use the Null-control Lentivirus (CAT#: CMV-Null-RB) as the negative control for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same selection marker expressed.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).