Pre-made inducible lentiviral particles for expressing human target: KCNJ5 (alternative name: CIR; GIRK4; KATP1; KIR3.4), 100% matched CDS region in NCBI ID: NM_000890.
Full sequence verified target is natively expressed under a tetracycline inducible suCMV promoter (Note: it can also used as regular constitutive high expression, see manual for details). A blasticidin-RFP fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual markers). RFP signal provides a convenient, realtime monitoring the particles performance. Each particles was validated in lot by lot basis and target expression is guaranteed. Particles were packaged in serum-free solution containing no any human or animal origin components. See product manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in serum-free medium.