This Premade lentivirus expresses human target, KDR (kinase insert domain receptor) (alternative name: CD309; FLK1; VEGFR; VEGFR2). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID:NM_002253.2
The target is expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression, by tetracycline or Dox induction, when the tetracycline repressor protein (TetR) is present.
It contains a RFP-Blasticidin fusion dual marker under the RSV promoter, which allows fluorescence sorting or blasticidin resistance selection of transduced cells. The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).