Pre-made lentivirus express human target: neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS), (alternative name: N-ras, RP5-1000E10.2, ALPS4, NRAS1, NS6), 100% matched CDS region in NCBI ID: NM_002524.
The target is natively expressed under a optional inducible CMV promoter (TetCMV) (Note: it can used as regular constitutive high expression, but optionally, can be used for inducible expression when its repressor, TetR present. It contains a RFP-Blasticidin fusion marker under RSV promoter, which allows to sort or select transduced cells via RFP signal or via blasticidin killing. RFP signal provides a convenient, realtime monitoring the particles performance.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium, containing 10x polybrene (60 ug/ml ).