Pre-made lentivirus express human target: h PTEN (alternative name: BZS; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_000314.4. Particles also contains a RFP-Blasticidin dual selection marker.
Full sequence verified target is natively expressed under a tetracycline optional inducible suCMV promoter (Note: it can used as regular constitutive high expression without any induction, and also as tetracycline inducible expression when desired). A RFP-Blasticidin fusion marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic (Dual markers). RFP signal provides a convenient, realtime monitoring the particles performance.
Each particles was validated in lot by lot basis and target expression is guaranteed. See Product manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium.