Lentivirus expresses human target: Sirt2 (sirtuin 2), (Gene_Synonym: SIR2; SIR2L; SIR2L2), with coding sequence 100% matching to CDS region in NCBI ID: NM_012237. It contains a RFP–Blasticidin dual selection marker.
The target was expressed under the optional inducible CMV promoter (TetCMV) (click to see more technical details). It constitutively expresses the target. Also optionally, It can be used for the inducible expression when used together with its repressor (TetR). An RFP-Blasticidin fusion dual selection marker under RSV promoter, allows to sort or select transduced cells via RFP signal or via blasticidin antibiotic. RFP signal provides convenient, realtime monitoring of the performance of the particles.
When desired, use the Null-control Lentivirus (CAT#: CMV-Null-RB) as the negative control for lentivirus treatment in your cell culture. The Null-Control does not express any targets, but has the same selection marker expressed.
See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).