Pre-made Lentivirus expressing human target: SNAI2 (alternative name: SLUG, SLUGH1, SNAIL2, WS2D) with a C-terminal HA tag. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID:NM_003068.
The target is expressed under an optional inducible suCMV promoter (Means: it can also be used as a regular constitutive high expression without need for any induction. But optionally, it can be used as tetracycline inducible expression when its repressor TetR is present). It carries the RFP-Blasticidin fusion dual selection marker under RSV promoter, which allows to sort or to select the transduced cells via RFP signal or via blasticidin antibiotic). RFP signal provides a convenient, realtime monitoring the lentivirus’s performance. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml, provided in DMEM medium containing 10x polybrene.